45. Which of the following sequence of the steps is regular for Western Blotting Assay that is widely used for detection of the specific protein in a tissue?

1. Tissues are broken down using a homogenizer or by sonication. 

 2. The protein is probed with an addition of the primary antibody.

 3. The protein is transferred to a PVDF membrane. 

 4. Add secondary antibody conjugated with peroxidase. 

 5. The proteins of the tissues are separated using gel electrophoresis.

6. The protein is detected with an addition of a peroxidase substrate.
(A) 123456
(B) 153246
(C) 152346
(D) 154623
(E) 124653

41. Which of the following normal ranges for measurements of some fasting blood values is FALSE? (A) Glucose: 75-110 mg/dL (B) Arterial blood pH: 7.00-7.45 (C) Protein: 6.5-8 g/dL (D) Cholesterol: less than 200 mg/dL (E) Sodium: 135-145 mM

42. Which of these statements about a secondary immune response is FALSE? (A) Antibodies of the IgE class are produced. (B) Antibodies are made quickly and in great amounts. (C) Antibody production lasts longer than in a primary response. (D) Lymphocyte clones are believed to develop. (E) It provides active immunity against the specific pathogens.

43. Which of these statements about muscle contraction is FALSE? (A) Ca2+ is required for all muscle contraction. (B) Troponin is present in skeletal muscles and smooth muscles. (C) When a skeletal muscle shortens during contraction, the H band and sarcomere shorten. (D) Skeletal muscles but not cardiac muscles require neural stimulation to contract. (E) The sarcoplasmic reticulum and transverse tubules are well-developed in skeletal muscles and poorly developed in smooth muscles. 

44. What is the correct sequence of events involving cyclic AMP as a second messenger of glucagon hormone?  1. The stimulation of the activity of adenylate cyclase.  2. The activation of G-proteins. 3. The conversion of ATP to cyclic AMP.  4. The activation of of protein kinase A.  5. The stimulation of lipid mobilization.  6. An increase in the activity of hormone-sensitive lipase. (A) 213645 (B) 213456 (C) 214356 (D) 213465 (E) 214365 

46. All are stages in gene transcription EXCEPT: (A) binding of RNA polymerase holoenzyme at the promoter sites. (B) DNase I activity on RNA polymerase/DNA complex. (C) chain elongation. (D) initiation of polymerization. (E) chain termination.

47. The driving force that pushes the root tip through the soil is primarily ______. (A) the elongation of root hairs (B) continuous cell division of root cap cells (C) elongation of cells behind the root apical meristem (D) continuous cell division in the root cap at the tip of the root (E) continuous cell division just behind the root cap in the center of the apical meristem

48. Iron deficiency is often indicated by yellowing in newly formed leaves. This suggests that iron ______. (A) is a relatively immobile nutrient in plants (B) is concentrated in the xylem of older leaves (C) is tied up in formed chlorophyll molecules (D) is concentrated in the phloem of older leaves (E) is found in leghemoglobin and reduces the amount available to new plant parts

49. You find a new species of mouse that, compared to the house mouse you are familiar with, sprints extraordinarily rapidly but also fatigues quickly. Which type of muscle fiber (compared to an ordinary house mouse) would you expect to see an increase in ______? (A) slow glycolytic fibers (B) slow oxidative fibers (C) fast glycolytic fibers (D) fast oxidative fibers (E) fast and slow oxidative fibers

50. Which of the following is true about short-term and long-term memory processes? (A) Both short-term and long-term memory processes require protein synthesis. (B) Neither short-term nor long-term memory processes require protein synthesis. (C) Invertebrate animals like Aplysia and Drosophila do not show long-term memory processes. (D) Short-term memory processes act through second messenger systems while long-term memory processes require protein synthesis. (E) Long-term memory processes act through second messenger systems while short-term memory processes require protein synthesis. .

51. An open circulatory system differs from a closed one in that: (A) The open circulatory system has a combination of blood vessels and large thin-walled sinuses whereas a closed circulatory system has only vessels. (B) An open circulatory system opens into the heart whereas the closed circulatory system does not. (C) An open circulatory system opens into arteries and is drained by veins whereas in a closed one it is just the reverse. (D) An open circulatory system drains into the mantle cavity whereas a closed circulatory system drains into the hemolymph. (E) A closed circulatory system is completely encased in the body cavity whereas the open one is not.