66. For treatment of AIDS, which is NOT a target for the approved drugs?
(A) CD4
(B) reverse transcriptase
(C) neuraminidase
(D) protease
(E) integrase

複選題62. What compound offers the one-carbon unit to produce N5 , N10 -methylene tetrahydrofolate in one carbon metabolism? (A) Methionine (B) Serine (C) S-Adenosyl methionine (D) Choline (E) Glycine

63. What enzyme(s) is required for the cleavage of the unmethylated strand during the mismatched repair? (A) MutL (B) MutH (C) MutS (D) DNA helicase II/exonuclease VII (E) Exonuclease I/exonuclease X

64. What domain(s) of regulatory proteins for gene regulation is involved in dimerization? (A) Helix-turn-helix (B) Helix-loop-helix (C) Zinc finger (D) Homeodomain (E) All of the above

65. Which statement for hemoglobin (the oxygen-binding protein in red blood cells) is FALSE? (A) Hemoglobin exists in T and R states and R state prefers to bind oxygen. (B) Oxygen binding converts hemoglobin from T to R state. (C) Oxygen binding to hemoglobin is both allosteric and cooperative. (D) Hemoglobin also binds H+ and CO2 to lessen the affinity for O2. (E) Oxygen binding is also regulated by 2,3-bisphosphoglycerate, which stabilizes R state.

67. Which of the following receptors and their types does NOT match? (A) Insulin receptor is a G-protein-coupled receptor. (B) Epidermal growth factor receptor is a receptor tyrosine kinase. (C) Acetylcholine receptor is a gated ion channel. (D) Integrin receptor is an adhesion receptor. (E) Steroid receptor is a nuclear receptor.

68. Which of the following compounds has the highest (the most negative) standard free energy of hydrolysis? (A) ATP (to ADP) (B) ADP (to AMP) (C) AMP (to adenosine) (D) Glucose 6-phosphate (to glucose) (E) Acetyl-CoA (to acetate)

69. Which of the following enzymatic cofactors and enzymes is NOT matched? (A) Biotin for pyruvate carboxylase (B) NAD+ for glucose 6-phosphate dehydrogenase (C) Pyridoxal phosphate (PLP) for aminotransferases (D) Thiamine pyrophosphate (TPP) for pyruvate decarboxylase (E) Vitamin B12 for methylmalonyl-CoA mutase

70. How many ATP can be net produced in a 14-carbon fatty acid degradation to CO2 and H2O? (A) 88 (B) 92 (C) 94 (D) 96 (E) 98

71. Fatty acids are carboxylic acids with hydrocarbon chains ranging from ________ carbons long. (A) 12 to 24 (B) 4 to 36 (C) 6 to 24 (D) 6 to 36 (E) 12 to 32

72. The molecular weight of Protein A (pI = 4.0), B (pI = 6.8), and C (pI = 8.5) is 14 kDa, 24 kDa, and 30 kDa, respectively. All of them are monomer proteins. Which of the following statements is CORRECT? (A) Protein A elutes out first, when you use gel filtration column to separate the mixture of those three proteins. (B) Protein C is the one that moves fastest, when you separate the mixture of those three proteins by SDS-PAGE. (C) As shown in the right figure, you can use this isoelectric focusing apparatus to separate those three proteins. (D) When you use a DEAE ion exchange column and 10 mM Tris-Cl, pH 7.0 buffer to separate these three proteins, Protein A will be bound by this column. (E) None of the above