69. Which of the following enzymatic cofactors and enzymes is NOT matched?
(A) Biotin for pyruvate carboxylase
(B) NAD+ for glucose 6-phosphate dehydrogenase
(C) Pyridoxal phosphate (PLP) for aminotransferases
(D) Thiamine pyrophosphate (TPP) for pyruvate decarboxylase
(E) Vitamin B12 for methylmalonyl-CoA mutase

65. Which statement for hemoglobin (the oxygen-binding protein in red blood cells) is FALSE? (A) Hemoglobin exists in T and R states and R state prefers to bind oxygen. (B) Oxygen binding converts hemoglobin from T to R state. (C) Oxygen binding to hemoglobin is both allosteric and cooperative. (D) Hemoglobin also binds H+ and CO2 to lessen the affinity for O2. (E) Oxygen binding is also regulated by 2,3-bisphosphoglycerate, which stabilizes R state.

66. For treatment of AIDS, which is NOT a target for the approved drugs? (A) CD4 (B) reverse transcriptase (C) neuraminidase (D) protease (E) integrase

67. Which of the following receptors and their types does NOT match? (A) Insulin receptor is a G-protein-coupled receptor. (B) Epidermal growth factor receptor is a receptor tyrosine kinase. (C) Acetylcholine receptor is a gated ion channel. (D) Integrin receptor is an adhesion receptor. (E) Steroid receptor is a nuclear receptor.

68. Which of the following compounds has the highest (the most negative) standard free energy of hydrolysis? (A) ATP (to ADP) (B) ADP (to AMP) (C) AMP (to adenosine) (D) Glucose 6-phosphate (to glucose) (E) Acetyl-CoA (to acetate)

70. How many ATP can be net produced in a 14-carbon fatty acid degradation to CO2 and H2O? (A) 88 (B) 92 (C) 94 (D) 96 (E) 98

71. Fatty acids are carboxylic acids with hydrocarbon chains ranging from ________ carbons long. (A) 12 to 24 (B) 4 to 36 (C) 6 to 24 (D) 6 to 36 (E) 12 to 32

72. The molecular weight of Protein A (pI = 4.0), B (pI = 6.8), and C (pI = 8.5) is 14 kDa, 24 kDa, and 30 kDa, respectively. All of them are monomer proteins. Which of the following statements is CORRECT? (A) Protein A elutes out first, when you use gel filtration column to separate the mixture of those three proteins. (B) Protein C is the one that moves fastest, when you separate the mixture of those three proteins by SDS-PAGE. (C) As shown in the right figure, you can use this isoelectric focusing apparatus to separate those three proteins. (D) When you use a DEAE ion exchange column and 10 mM Tris-Cl, pH 7.0 buffer to separate these three proteins, Protein A will be bound by this column. (E) None of the above

73. Which of the following is NOT related to RNA-RNA interaction? (A) guide RNA (B) Wobble (C) Shine-Dalgarno sequence (D) snRNA (E) None of the above

74. (1) Arg； (2) Asp； (3) Gly； (4) Cys； (5) His； (6) Ser； (7) Thr. Which of the followings does NOT appear in the preceding seven amino acid residues? (A) The amino acid residue in zinc finger to coordinate zinc atom (B) The amino acid residue in O-link glycoprotein to link protein and oligosaccharide (C) The amino acid residue in Type I topoisomerase to act as a nucleophile to cut DNA (D) The amino acid residue in terminal proteins of adenovirus to link nucleotides and protein (E) None of the above

75. Which of the following compounds is the substrate of ribonucleotide reductase? (A) AMP (B) TDP (C) GTP (D) CDP (E) dAMP