試題詳解

28. Which statement incorrectly describes the application of Next-Generation Sequencing (NGS) and Single-cell RNA sequencing (scRNA-seq) technologies?
(A) Size selection during library preparation in NGS is crucial for ensuring the uniformity of fragment lengths, which impacts the efficiency and accuracy of sequencing.
(B) Fragmentation is not a concern in NGS as modern sequencing technologies can accurately reconstruct entire genomes from small DNA fragments.
(C) NGS-based transcriptomic profiling can accurately quantify gene expression levels, but it is unable to capture post-transcriptional modifications such as RNA editing events.
(D) scRNA-seq uncovers the dynamics of immune cell activation and differentiation in response to cancer immunotherapy.
(E) Both NGS and scRNA-seq are critical in the field of evolutionary editing events.