29. Long noncoding RNAs (lncRNAs) are described as long transcripts more than 200 nucleotides, lacking protein-coding potential. LncRNAs participate in regulation of gene expression at multiple levels via interacting with DNA, RNA and proteins.
(A) LncRNAs are transcribed by RNA pol II but no intron, no cap and no poly（Ａ） tail.
(B) LncRNAs have high expression levels.
(C) LncRNAs are more enriched in the cytoplasm.
(D) LncRNAs recruit PRC2 complex and lead to H3K27 methylation for gene inhibition.
(E) All are correct.

25. Which chromatography method would best separate proteins based on their isoelectric points (pI)? (A) Ion exchange chromatography (B) Gel filtration chromatography (C) Reverse-phase chromatography (D) Affinity chromatography (E) IMAC (Immobilized metal affinity chromatography)

26. To repair the insertion/deletion created by the Crispr/Cas9 editing mainly needs: (A) Base excision repair (B) Nuclear excision repair (C) Mismatch repair (D) Homologous recombination (E) Non-homologous end-joining

27. Antibody-dependent cytotoxicity will evoke: (A) cell proliferation (B) auto-immune response. (C) Epidermal-mesenchymal transition (D) apoptosis of cancer cells (E) activation of PI3K/Akt signaling

28. The molecular mechanism of the gene expression revealed the role of specific transcription factors, promoters, enhancers, and cofactors involved. Which experiment is incorrect? (A) The transcription factor-binding sequences can be determined with chromatin-immunoprecipitation (IP) followed by DNA sequencing. (B) The promoter and enhancer were cloned from the cDNA library for the reporter assay. (C) The transcription factor was knocked down by siRNA for the loss-of-function analysis. (D) The transcription factor was overexpressed with a viral vector for the gain-of-function analysis. (E) The transcription factor-associated cofactors were identified by the IP-Mass Spectrometry (MS) analysis.

30. The 2024 Nobel Prize in Physiology or Medicine was awarded to Victor Ambros and Gary Ruvkun for discovering microRNA and its role in post-transcriptional gene regulation. Which statement is incorrect? (A) Lin-4 was the first microRNA to be discovered by Victor Ambros's team. (B) Victor Ambros's team found that lin-4 negatively regulates LIN-14 protein expression. (C) Gary Ruvkun's team found the 5'UTR of lin-14 mRNA is required for lin-4 inhibition. (D) In C . elegans, the microRNA lin-4 critically regulates developmental timing (E) Most microRNAs are conserved across species.

31. There is no codon for the amino acid hydroxyproline, but this amino acid is a prominent feature of collagen structure. Which of the following is a likely explanation? (A) Hydroxyproline is substituted for proline after translation by a cut and patch mechanism. (B) Proline is covalently modified to give hydroxyproline after translation. (C) There is an alternative mechanism for synthesis of proteins that contain hydroxyproline. (D) All of above are likely explanations. (E) It is not possible to form a hypothesis from the information given.

32. One of the hopes for use of recent knowledge gained about non-coding RNAs lies with the possibilities for their use in medicine. Of the following scenarios for future research, which would you expect to gain most from RNAs? (A) Exploring a way to turn on the expression of pseudogenes. (B) Targeting siRNAs to disable the expression of an allele associated with autosomal recessive disease. (C) Targeting siRNAs to disable the expression of an allele associated with autosomal dominant disease. (D) Creating knock-out organisms that can be useful for pharmaceutical drug design. (E) Looking for a way to prevent viral DNA from causing infection in humans.

33. Which of the following statement regarding bacterial artificial chromosomes (BACs) is correct? (A) BACs are generally not stable. (B) BACs are present in high copy numbers per cell. (C) BACs are derived from the E. coli chromosome. (D) BACs can maintain DNA sequences of hundreds of thousands of nucleotide pairs. (E) All of the above.

34. Which of the following descriptions regarding cDNA libraries is correct? (1) The production of cDNA requires use of reverse transcriptase.  (2) cDNA contain intronic sequences. (3) cDNA libraries are enriched in protein-coding genes.  (4) cDNA libraries are cell-type specific. (A) (1) and (2) (B) (1), (3), and (4) (C) (1) only (D) (2), (3), and (4) (E) All of the descriptions are correct.

35. Which of the following descriptions regarding the electrical properties of cellular membranes is correct? (A) If the membrane is impermeable to an ion, the membrane potential approaches the equilibrium potential for that ion. (B) The resting potential in most animal cells is between 20 mV and 120 mV (positive inside). (C) Setting up (or changing) the membrane potential requires changing the bulk concentrations of the ions on the two sides of the membrane. (D) The resting potential decays immediately following the inhibition of the Na+-K+ pump by a drug. (E) None of the above.