45.ClpB is an ATPase, functioning as a chloroplastic disaggregase. Biochemical characterization, including the SDS-PAGE and gel filtration chromatography of the purified ClpB, was performed to understand this enzyme better. Which of the following statements is most likely?
(A) The functional form of ClpB is a hexamer.
(B) ClpB is 2 monomer in solution.
(C) Mg+2 and ATP disaggregate ClpB.
(D) The molecular weight ofCIpB monomer is 200 kDa.
(E) In the presence of Mg+2 and ATP, CIpB displays an ordered tertiary structure.
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統計: A(2), B(1), C(0), D(2), E(1) #3236578
統計: A(2), B(1), C(0), D(2), E(1) #3236578
詳解 (共 1 筆)
#6348331
為了這題我還跑回去看他的原本論文,感覺題目沒有寫得很清楚,去看原文比較能看懂再說什麼
他fig1. m是molecular weight markers;1是The soluble cell extract from the induced bacterial culture
所以知道這個東西大概97kDa
然後fig2可以看到它可以有200kDa東西,所以他可以有2聚體
然後fig3可以看到有660多有最大值,所以他可以有6聚體
附上原文網址
附上原文網址
https://link.springer.com/article/10.1007/s11103-020-01050-7
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