試題詳解

48.SiR-tubulin is a microtubule dye used for visualizing microtubule dynamics in live cells, but cannot be used to stain paraformaldehyde (PFA﹚ and methanol-fixed cells. Why is this dye unsuitable for fixed cells?
(A) These fixation methods alter the structure of the SiR-tubulin binding site on microtubules.
(B) The pH of fixed cells is not optimal for SiR-tubulin binding.
(C) The SiR-tubulin is a crosslinker of microtubules. PFA and methanol interfere with the reactivity ofthe dye﹒
(D) PFAand mcthanol prevent dye penetration﹒
(E) PFAand methanol significantly quench the fluorescence signal of the dye﹒