試題詳解

49. Electrophoresis mobility shift assay (EMSA) is a sensitive method to detect protein-DNA interactions. The proteins and fluorescent DNA were combined as indicated and electrophoresed under native conditions, as shown below. Which of the following statements would be most likely?
 
(A) Protein B binds to the DNA independent of protein A.
(B) Protein A binds to the DNA only in the presence of protein B.
(C) Protein A and protein B compete for DNA binding.
(D) Protein A and protein B form a protein complex before binding to the DNA.
(E) Protein B binds to the protein A-DNA complex, resulting in a ternary complex.