試題詳解

52. Which of the following statements about the initial step of the reaction catalyzed by thermolysin is NOT correct?

(A) The enzyme reaction involves the general acid-base catalysis.
(B) A glutamate residue deprotonates water and promotes hydroxide attack on the carbonyl carbon of the substrate.
(C) The ion stabilizes the buildup of negative charge on the peptide carbonyl oxygen.
(D) Collapse of the unstable tetrahedral intermediate links the substrate to the glutamate residue covalently.
(E) The enzyme is a member of hydrolases.