56. Glutamate dehydrogenase catalyzes the reaction shown below. In a reaction mixture containing a saturated quantity of glutamate and NAD+, how many units (U) of glutamate dehydrogenase should be added to increase the absorption at 340 nm by 1.0 OD in one minute? Please note that the molar extinction coefficient (ε) of NADH is 6220 liter/mol/cm at 340 nm, and the volume ofthe reaction mixture is 1 ml.

 
(A)16U
(B)1.6U
(C)0.16 U
(D) 0.016 U
(E) 0.0016 U

答案:登入後查看
統計: A(0), B(1), C(6), D(0), E(0) #3236589

詳解 (共 1 筆)

#6261280
首先用Beer's law計算出NADH...
(共 330 字,隱藏中)
前往觀看
4
0