35. Protein acetylation is an important mode of regulation. Which of the following statements about reversible protein acetylation is incorrect? (A) Protein acetylation usually uses acetyl-CoA as the acetyI group donor. (B) The deacetylation reaction often involves the participation of NAD+ as a cofactor. (C) The reaction typically occurs at the a-amino group of a lysine in the target (D) Almost all enzymes in the TCA cycle can be regulated by protein acetylation (E) Sirtuin-3 is a mitochondrial deacetylase that activates a TCA cycle enzyme - isocitrate dehydrogenase. protein.